ProtumLink Union 50cm

The 50 cm column is the most resolving option in the series, intended for the highest-depth analyses, where maximum peak capacity and sensitivity are required.

Measured at 250 nL/min, the column achieves ~5.7 s FWHM over ~95 min gradients, offering the separation space necessary to distinguish low-abundance and closely eluting peptides. This is particularly impactful for PTM mapping, deep clinical proteomics, and complex structural studies.

With an operational range of 100–400 nL/min, the 50 cm column performs reliably over long analytical sequences and requires stable UHPLC pressure capability. It is the preferred option when analytical depth, precision, and confidence take priority over throughput.

Union columns feature an integrated liquid junction that enables straightforward connection to nano-LC systems using standard 10-32 threaded fittings. This configuration provides a robust, zero-dead-volume interface and allows fast column exchange without additional adapters. The integrated junction can also serve as the grounding or high-voltage application point when required by the instrument configuration. Union columns are fully compatible with setups using Thermo Scientific Nanospray Flex ion sources, where the high voltage can be applied through the liquid connection. This standardized connectivity enables a direct and reliable link between the column outlet and the UHPLC system while preserving excellent chromatographic performance. Specific adapter configurations may be required depending on the vendor ion source when operating emitter-integrated columns with a pre-mounted liquid junction. Users should consult instrument documentation where compatibility with blank-emitter columns is explicitly described.

• Thermo Scientific nanoViper™ Fingertight Fittings • IDEX Health & Science MarvelXACT™ UHPLC Connection Systems • Waters ZenFit™ Connection Technology (compatibility pending verification)