ProtumLink Union Series
ProtumLink Union 25cm
Product Description
Deep Proteome Discovery
Core Benefit: High Resolution for Complex Proteomes
The 25 cm column is developed for researchers requiring higher resolving power to confidently analyze complex or fractionated samples.
Chromatographic Excellence (FWHM Resolution)
At 400 nL/min, FWHM values of ~4.2–5.5 s across 30–60 min gradients offer the extended separation necessary to reduce co-elution and co-isolation, which directly enhances identification depth and quantification quality. This format is particularly effective for biomarker discovery and deep profiling applications.
Backpressure Flexibility & Workflow Versatility
Operating within 100–800 nL/min, the 25 cm column is optimized for nanoflow high-resolution separations on UHPLC systems. It provides deeper biological insight without requiring excessively long runs, making it suitable for advanced discovery and complex experimental designs.
Pressure profiles
Compatibilities
Union columns feature an integrated liquid junction that enables straightforward connection to nano-LC systems using standard 10-32 threaded fittings. This configuration provides a robust, zero-dead-volume interface and allows fast column exchange without additional adapters. The integrated junction can also serve as the grounding or high-voltage application point when required by the instrument configuration. Union columns are fully compatible with setups using Thermo Scientific Nanospray Flex ion sources, where the high voltage can be applied through the liquid connection. This standardized connectivity enables a direct and reliable link between the column outlet and the UHPLC system while preserving excellent chromatographic performance. Specific adapter configurations may be required depending on the vendor ion source when operating emitter-integrated columns with a pre-mounted liquid junction. Users should consult instrument documentation where compatibility with blank-emitter columns is explicitly described.
Compatible Connection Systems
• Thermo Scientific nanoViper™ Fingertight Fittings • IDEX Health & Science MarvelXACT™ UHPLC Connection Systems • Waters ZenFit™ Connection Technology (compatibility pending verification)
C18 Bead Specification
Specification Table
| Property | Specification |
|---|---|
| Silica Brand | ReproSil-Pur 120 C18-AQ (Dr. Maisch GmbH) |
| Chromatographic Mode | Reversed-phase |
| Particle Size | 1.9 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m²/g |
| Bonding / Phase | C18 (ODS), fully end-capped, aqueous-compatible |
| Carbon Load | 15% |
| pH Stability Range | 2 – 8 |
| Max Operating Pressure | ≤ 1200 bar |
| Max Operating Temperature | 60 °C* |
*Ultra-fast gradients may be operated up to 70 °C.
Why We Use a Trusted C18 Phase
We rely on a well-established C18 material from Dr. Maisch because it has been widely used in LC–MS–based proteomics for nearly two decades and is known for its stable bonding chemistry, consistent material quality, and reproducible performance. This makes it a dependable foundation for achieving high-quality peptide separations across diverse workflows. The 1.9 µm C18 particles provide a balanced combination of peak capacity, mechanical robustness, and retention stability, supporting sharp and predictable peak shapes under the aqueous mobile-phase conditions typical for proteomics.
Application Notes
Ultra robust performance stability of 25 cm × 75 µm Emitter-Integrated Nanoflow Column with 1.9 µm C18 Material over 800 Samples
PDF - 1.5 MB
High-Sensitivity and Reproducible DIA Performance of a 15 cm × 75 µm Emitter-Integrated Nanoflow Column on the Orbitrap Astral System
PDF - 1.1 MB
Packages
Column ID (Diameter)