ProtumLink Extend Series
ProtumLink Extend 5cm
Product Description
High-Throughput Speed & Sensitivity
Core Benefit: Fast Discovery Proteomics
The ProtumLink 5 cm emitter-integrated column is designed for laboratories prioritizing high sampling throughput while maintaining the sensitivity needed for limited-input material. It is a reliable choice for rapid discovery and scouting experiments.
Chromatographic Excellence (FWHM Resolution)
Even under accelerated conditions, the 5 cm format maintains sharp and stable peak profiles. At 1 µL/min, FWHM values remain consistently in the 3.1–4.1 s range across 5–15 min gradients, supporting confident peptide quantification and robust detection in high-volume workflows and QC environments.
Backpressure Flexibility & Workflow Versatility
With a usable flow range from 100 nL/min (nanoflow) up to 2000 nL/min (capillary flow), the column adapts well to different analytical needs. Its low backpressure enables operation at up to 1 µL/min even on pressure-limited systems, making it easy to transition between rapid screens, QC checks, and sensitive low-input analyses without changing hardware.
Pressure profiles
Compatibilities
Extend columns are supplied in a Thermo EasySpray–compatible cassette format, enabling direct installation on EasySpray ion sources without additional emitter hardware or external high-voltage components. Electrical contact and emitter alignment are established automatically through the cassette interface. The Extend format follows widely used heater geometries, enabling compatibility with common external column heating systems. At the inlet side, an integrated liquid junction allows straightforward connection to nano-LC systems using standard 10-32 threaded female fittings, enabling fast column exchange while preserving zero-dead-volume performance. This configuration supports reliable nanospray performance, easy system setup, and seamless operation on Thermo Orbitrap platforms equipped with EasySpray sources.
Compatible Connection Systems
• Thermo Scientific nanoViper™ Fingertight Fittings • IDEX Health & Science MarvelXACT™ UHPLC Connection Systems • Waters ZenFit™ Connection Technology (compatibility pending verification)
C18 Bead Specification
Specification Table
| Property | Specification |
|---|---|
| Silica Brand | ReproSil-Pur 120 C18-AQ (Dr. Maisch GmbH) |
| Chromatographic Mode | Reversed-phase |
| Particle Size | 1.9 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m²/g |
| Bonding / Phase | C18 (ODS), fully end-capped, aqueous-compatible |
| Carbon Load | 15% |
| pH Stability Range | 2 – 8 |
| Max Operating Pressure | ≤ 1200 bar |
| Max Operating Temperature | 60 °C* |
*Ultra-fast gradients may be operated up to 70 °C.
Why We Use a Trusted C18 Phase
We rely on a well-established C18 material from Dr. Maisch because it has been widely used in LC–MS–based proteomics for nearly two decades and is known for its stable bonding chemistry, consistent material quality, and reproducible performance. This makes it a dependable foundation for achieving high-quality peptide separations across diverse workflows. The 1.9 µm C18 particles provide a balanced combination of peak capacity, mechanical robustness, and retention stability, supporting sharp and predictable peak shapes under the aqueous mobile-phase conditions typical for proteomics.
Application Notes
Ultra robust performance stability of 25 cm × 75 µm Emitter-Integrated Nanoflow Column with 1.9 µm C18 Material over 800 Samples
PDF - 1.5 MB
High-Sensitivity and Reproducible DIA Performance of a 15 cm × 75 µm Emitter-Integrated Nanoflow Column on the Orbitrap Astral System
PDF - 1.1 MB
Packages
Column ID (Diameter)