ProtumLink Extend 50cm

The 50 cm column is the most resolving option in the series, intended for the highest-depth analyses, where maximum peak capacity and sensitivity are required.

Measured at 250 nL/min, the column achieves ~5.7 s FWHM over ~95 min gradients, offering the separation space necessary to distinguish low-abundance and closely eluting peptides. This is particularly impactful for PTM mapping, deep clinical proteomics, and complex structural studies.

With an operational range of 100–400 nL/min, the 50 cm column performs reliably over long analytical sequences and requires stable UHPLC pressure capability. It is the preferred option when analytical depth, precision, and confidence take priority over throughput.

Extend columns are supplied in a Thermo EasySpray–compatible cassette format, enabling direct installation on EasySpray ion sources without additional emitter hardware or external high-voltage components. Electrical contact and emitter alignment are established automatically through the cassette interface. The Extend format follows widely used heater geometries, enabling compatibility with common external column heating systems. At the inlet side, an integrated liquid junction allows straightforward connection to nano-LC systems using standard 10-32 threaded female fittings, enabling fast column exchange while preserving zero-dead-volume performance. This configuration supports reliable nanospray performance, easy system setup, and seamless operation on Thermo Orbitrap platforms equipped with EasySpray sources.

• Thermo Scientific nanoViper™ Fingertight Fittings • IDEX Health & Science MarvelXACT™ UHPLC Connection Systems • Waters ZenFit™ Connection Technology (compatibility pending verification)