ProtumLink Core Series
ProtumLink Core 8cm
Product Description
Accelerated Quantitative Profiling
Core Benefit: Enhanced Separation in Medium-Throughput Workflows
The 8 cm format provides additional resolving power over shorter columns while still supporting the speed needed for routine quantitative proteomics.
Chromatographic Excellence (FWHM Resolution)
Measured at 800 nL/min, the column delivers consistent peak widths of ~3.4–4.2 s across 10–20 min gradients, offering focused peak geometry that improves duty-cycle efficiency and quantification reliability. This makes the format well suited for time-sensitive cohort studies and routine translational workflows.
Backpressure Flexibility & Workflow Versatility
An operational flow window of 100–1500 nL/min allows smooth movement between accelerated profiling and nanoflow-level sensitivity without system changes. The 8 cm length provides a practical balance of speed, resolving power, and robustness, making it suitable for laboratories running a broad mix of LC-MS assays.
Pressure profiles
Compatibilities
Core columns are bare fused-silica emitter-integrated analytical columns designed to be connected via a liquid junction. With an outer diameter of 363 μm, they are compatible with liquid junctions engineered for standard 360 μm fused-silica tubing. Depending on the instrument setup, metal junctions can provide grounding or high-voltage transfer, while PEEK junctions may be used when an external grounding or voltage supply point is available. Core columns can also be mounted directly in nanospray sources that are designed to accept bare emitter tips. This includes Thermo Scientific Nanospray Flex and Bruker CaptiveSpray sources (with appropriate emitter positioning accessories such as the CaptiveSpray (CSI) emitter adjustment kit), enabling use of the column itself as the electrospray emitter and minimizing post-column volume. Specific adapter configurations may be required depending on the vendor ion source when using bare emitter columns. Users should consult instrument documentation where compatibility with blank-emitter columns is explicitly described.
Compatible Connection Systems
• Thermo Scientific 6041.5290 nanoViper Fingertight Fitting System, 20 μm
Recommended Liquid Junction Options
• IDEX UH-750 VHP MicroTee® • IDEX UH-634 VHP MicroTight®
C18 Bead Specification
Specification Table
| Property | Specification |
|---|---|
| Silica Brand | ReproSil-Pur 120 C18-AQ (Dr. Maisch GmbH) |
| Chromatographic Mode | Reversed-phase |
| Particle Size | 1.9 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m²/g |
| Bonding / Phase | C18 (ODS), fully end-capped, aqueous-compatible |
| Carbon Load | 15% |
| pH Stability Range | 2 – 8 |
| Max Operating Pressure | ≤ 1200 bar |
| Max Operating Temperature | 60 °C* |
*Ultra-fast gradients may be operated up to 70 °C.
Why We Use a Trusted C18 Phase
We rely on a well-established C18 material from Dr. Maisch because it has been widely used in LC–MS–based proteomics for nearly two decades and is known for its stable bonding chemistry, consistent material quality, and reproducible performance. This makes it a dependable foundation for achieving high-quality peptide separations across diverse workflows. The 1.9 µm C18 particles provide a balanced combination of peak capacity, mechanical robustness, and retention stability, supporting sharp and predictable peak shapes under the aqueous mobile-phase conditions typical for proteomics.
Application Notes
Ultra robust performance stability of 25 cm × 75 µm Emitter-Integrated Nanoflow Column with 1.9 µm C18 Material over 800 Samples
PDF - 1.5 MB
High-Sensitivity and Reproducible DIA Performance of a 15 cm × 75 µm Emitter-Integrated Nanoflow Column on the Orbitrap Astral System
PDF - 1.1 MB
Packages
Column ID (Diameter)