ProtumLink Connect Series
ProtumLink Connect 8cm
Product Description
Accelerated Quantitative Profiling
Core Benefit: Enhanced Separation in Medium-Throughput Workflows
The 8 cm format provides additional resolving power over shorter columns while still supporting the speed needed for routine quantitative proteomics.
Chromatographic Excellence (FWHM Resolution)
Measured at 800 nL/min, the column delivers consistent peak widths of ~3.4–4.2 s across 10–20 min gradients, offering focused peak geometry that improves duty-cycle efficiency and quantification reliability. This makes the format well suited for time-sensitive cohort studies and routine translational workflows.
Backpressure Flexibility & Workflow Versatility
An operational flow window of 100–1500 nL/min allows smooth movement between accelerated profiling and nanoflow-level sensitivity without system changes. The 8 cm length provides a practical balance of speed, resolving power, and robustness, making it suitable for laboratories running a broad mix of LC-MS assays.
Pressure profiles
Compatibilities
Connect columns feature a pre-installed CaptiveSpray Inlet (CSI) adapter at the emitter end, enabling direct use on Bruker CaptiveSpray and CaptiveSpray Ultra ion sources without additional emitter hardware. At the inlet side, an integrated liquid junction allows straightforward connection to nano-LC systems using standard 10-32 threaded female fittings, enabling fast column exchange and preserving zero-dead-volume performance. In Bruker configurations, grounding is applied through the liquid junction, ensuring proper electrical contact and stable electrospray operation. This configuration provides a robust nanospray interface with minimal post-column dispersion, stable spray performance, and seamless integration with Bruker timsTOF platforms.
Compatible Connection Systems
• Thermo Scientific nanoViper™ Fingertight Fittings • IDEX Health & Science MarvelXACT™ UHPLC Connection Systems • Waters ZenFit™ Connection Technology (compatibility pending verification)
C18 Bead Specification
Specification Table
| Property | Specification |
|---|---|
| Silica Brand | ReproSil-Pur 120 C18-AQ (Dr. Maisch GmbH) |
| Chromatographic Mode | Reversed-phase |
| Particle Size | 1.9 µm |
| Pore Size | 120 Å |
| Surface Area | 300 m²/g |
| Bonding / Phase | C18 (ODS), fully end-capped, aqueous-compatible |
| Carbon Load | 15% |
| pH Stability Range | 2 – 8 |
| Max Operating Pressure | ≤ 1200 bar |
| Max Operating Temperature | 60 °C* |
*Ultra-fast gradients may be operated up to 70 °C.
Why We Use a Trusted C18 Phase
We rely on a well-established C18 material from Dr. Maisch because it has been widely used in LC–MS–based proteomics for nearly two decades and is known for its stable bonding chemistry, consistent material quality, and reproducible performance. This makes it a dependable foundation for achieving high-quality peptide separations across diverse workflows. The 1.9 µm C18 particles provide a balanced combination of peak capacity, mechanical robustness, and retention stability, supporting sharp and predictable peak shapes under the aqueous mobile-phase conditions typical for proteomics.
Application Notes
Ultra robust performance stability of 25 cm × 75 µm Emitter-Integrated Nanoflow Column with 1.9 µm C18 Material over 800 Samples
PDF - 1.5 MB
High-Sensitivity and Reproducible DIA Performance of a 15 cm × 75 µm Emitter-Integrated Nanoflow Column on the Orbitrap Astral System
PDF - 1.1 MB
Packages
Column ID (Diameter)